Mubarakpuri began his studies at home with the Quran under the tutelage of his grandfather and uncle. He then began studies in Arabic and Persian after getting admission in Madrasa Arabia Dar-ut-Taleem. He later moved to Madrasah Ihyaaul Uloom in Mubarakpur after being admitted there in 1954. Two years after that he joined Madrasa Faiz-e-Aam Maunath Bhanjan (Mau district) for further studies. Upon completion of his seven years of studies, he acquired the Fadilat degree and passed multiple exams to receive the Maulvi and Alim Certification. Mubarakpuri then went on to teach for the next 28 years in various universities, madrasas, and schools in India until he was offered a position at the Islamic University of Al Madinah al-Munawarah. He served several years at the Islamic University of Madinah.